Q1. Which of the following statements accurately reflects the principle, application, and limitations of DNA barcoding in biodiversity assessment and species identification?
- (a) DNA barcoding uses the full genome of an organism to identify species with 100% accuracy, making it a superior method to all traditional taxonomic techniques for both plants and animals.
- (b) DNA barcoding relies on sequencing a short, standardized region of the genome—such as the COI gene in animals or rbcL/matK in plants—to identify and classify species, but its effectiveness may be limited by lack of reference databases and low resolution in closely related species
- (c) DNA barcoding is limited only to microbial species and uses protein-coding genes to distinguish between strains, and thus has little relevance in conservation biology or forest management.
- (d) DNA barcoding eliminates the need for morphological or ecological data and is currently used as the sole identification tool in all global biodiversity conventions and trade regulations.
Answer & solution
Answer: (b) DNA barcoding relies on sequencing a short, standardized region of the genome—such as the COI gene in animals or rbcL/matK in plants—to identify and classify species, but its effectiveness may be limited by lack of reference databases and low resolution in closely related species
A DNA barcode is a short, standard gene fragment, not the whole genome, matched against a reference library to name the species.
It is a complement to classical taxonomy, and it fails where the library is incomplete or where two species diverged too recently to differ in that fragment.
Revision note — DNA barcoding
• Proposed by Paul Hebert of the University of Guelph, Canada, in 2003; the idea is that one short sequence can identify a species the way a supermarket barcode identifies a product.
• Animals: about 650 base pairs of the mitochondrial gene COI (cytochrome c oxidase subunit I).
• Plants: the chloroplast genes rbcL and matK. Fungi: the nuclear ITS (internal transcribed spacer) region.
• Reference sequences are stored in BOLD (Barcode of Life Data System) and GenBank; an unknown sample is sequenced, compared and assigned to the closest match.
• Uses: wildlife forensics (seized ivory, shark fins, timber), food fraud (mislabelled fish), quarantine pests, and environmental DNA (eDNA) surveys of rivers and soils.
• Limits: incomplete reference databases, low resolution among closely related or recently diverged species, hybrids, and mitochondrial DNA being inherited only from the mother.
• In India the Zoological Survey of India (1916) and the Botanical Survey of India (1890) build the barcode libraries.
• The Biological Diversity Act, 2002 set up the National Biodiversity Authority at Chennai.
Do not mix these up
• DNA barcoding vs DNA fingerprinting — barcoding identifies a species from a standard gene; fingerprinting (Alec Jeffreys, 1984) tells apart individuals of one species, as in paternity and crime cases.
• Barcoding vs whole-genome sequencing — a barcode reads one short fragment; genome sequencing reads all the DNA and is far costlier.
Also asked from this topic: the main objective of the Genome India Project (to map the genetic diversity of Indians); what a gene is (a section of DNA that codes for a protein).